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Wednesday, May 28, 2025
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Cloning

assay development

Amplified insert assembly-PDF

Overview This is a lab specific protocol. For more information on the benefits of Amplified Insert Assembly see the consensus protocol. A You-Tube video summary...
gene therapy & gene editing

Making a multi-part plasmid-V1-S2-PDF

Overview This protocol describes how to assemble multiple-part plasmids via BsaI digestion and T7 Ligase ligation. It is intended to supplement Lee, M. E., DeLoache,...

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